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Is PKC Playing a Role in the Tolerance to
Ischemia of Spontaneously
Hypertensive Rats (SHR)?

Mosca, Susana María;
Cingolani, Horacio Eugenio

Centro de Investigaciones Cardiovasculares, Facultad de
Ciencias Médicas, Universidad Nacional de La Plata,
La Plata, Argentina

   Previous works in normotensive animals demonstrated that protein-kinase C (PKC) activation improves postischemic recovery of myocardium. Our objective was to determine if PKC participates in the tolerance to ischemia of SHR. Isolated perfused hearts from SHR and Wistar Kyoto (WKY) normotensive rats were submitted to a 20-min global ischemic period (Is) followed by 30 min of reperfusion (R). This protocol was repeated in presence of PKC blocker, chelerythrine (Ch) 25mg/min. The postischemic recovery and ischemic contracture (IC) were assessed through the left ventricular developed pressure (LVDP) and end diastolic pressure (LVEDP), respectively. At the end of R, LVDP recovered 65 ± 11% and 64 ± 9% respect to baseline values in control hearts of SHR and WKY, respectively. In the presence of Ch the postischemic recovery was significantly lesser than in control conditions (LVDP was 26 ± 12% and 29 ± 12% in SHR and WKY, respectively). At the end of ischemic period, LVEDP was 37 ± 6 mmHg and 29 ± 4 mmHg for SHR and WKY, respectively and diminished significantly after Ch administration (LVEDP= 10 ± 4 mmHg for both strains).

   We concluded that: 1) the response to ischemia is similar for SHR and WKY rats and 2) the PKC inhibition increased the postischemic dysfunction in a similar degree in both strains, suggesting that PKC activation is participating in the protection against ischemia in these animals.



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2nd Virtual Congress of Cardiology

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